Evaluation of combined use of the MALDI-TOF and GenomEra MRSA/SA assay for the direct detection of methicillin resistance in Staphylococcus aureus from positive blood culture bottles.
نویسندگان
چکیده
Months D–F S–D D–F D–F D–F D–F D–F Patients 229 341 314 248 206 117 157 RSV 121 102 99 121 87 68 89 Rhinovirus 64 118 63 56 61 27 44 Human bocavirus 24 41 45 33 16 7 7 Adenovirus 17 28 43 16 17 8 19 Parainfluenza (1, 2, 3, 4) 15 40 7 7 7 4 3 Human metapneumovirus 8 0 6 8 1 1 6 Influenza (A, B, C) 28 38 24 14 14 7 10 Enterovirus 9 7 2 5 7 1 4 Coronavirus 2 6 1 12 2 0 5 Negative 34 69 98 51 49 18 29
منابع مشابه
One-step sample preparation of positive blood cultures for the direct detection of methicillin-sensitive and -resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci within one hour using the automated GenomEra CDXTM PCR system
A method for the rapid detection of methicillinsensitive and -resistant Staphylococcus aureus (MSSA and MRSA, respectively) and methicillin-resistant coagulasenegative staphylococci (MRCoNS) with a straightforward sample preparation protocol of blood cultures using an automated homogeneous polymerase chain reaction (PCR) assay, the GenomEraTM MRSA/SA (Abacus Diagnostica Oy, Turku, Finland), is ...
متن کاملGenomEra MRSA/SA, a fully automated homogeneous PCR assay for rapid detection of Staphylococcus aureus and the marker of methicillin resistance in various sample matrixes.
The GenomEra MRSA/SA assay (Abacus Diagnostica, Turku, Finland) is the first commercial homogeneous PCR assay using thermally stable, intrinsically fluorescent time-resolved fluorometric (TRF) labels resistant to autofluorescence and other background effects. This fully automated closed tube PCR assay simultaneously detects Staphylococcus aureus specific DNA and the mecA gene within 50 min. It ...
متن کاملRapid detection of methicillin-sensitive and resistanct Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococci from blood cultures by automated PCR assay
Introduction / objectives We assessed the usefulness of a new automated molecular assay (GenomEra MRSA Confirm, Abacus Diagnostica Oy, Finland), which amplifies simultaneously the marker of methicillin resistance (mecA) and a Staphylococcus aureus-specific DNA segment, in detection of methicillin sensitive and resistant S. aureus (MSSA and MRSA) and other methicillin resistant staphylococci (MR...
متن کاملRapid PCR detection of methicillin-resistant Staphylococcus aureus and methicillin-sensitive S. aureus samples from charcoal-containing blood culture bottles.
Recent advances in technology have allowed development of automated, rapid, real-time PCR tests that are able to differentiate between methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in blood cultures. These include the Cepheid Xpert MRSA/SA blood culture assay used with the GeneXpert Dx system (Cepheid, Sunnyvale, CA), which has been found to have ...
متن کاملEvaluation of the BD GeneOhm StaphSR assay for detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from spiked positive blood culture bottles.
To improve the clinical outcome of Staphylococcus aureus septicemia, the early selection of appropriate antibiotic treatment is crucial. Molecular diagnostics represents an attractive approach for the rapid identification of S. aureus and the determination of its methicillin (meticillin) resistance. In direct comparison to other molecular assays (sa442 and mecA real-time PCRs) and standard labo...
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ورودعنوان ژورنال:
- Enfermedades infecciosas y microbiologia clinica
دوره 34 10 شماره
صفحات -
تاریخ انتشار 2016